Scientists Develop New Gene Therapy Strategy to Delay Aging and Extend Lifespan


Reverse Aging

Cellular senescence, a state of permanent growth arrest, has emerged as a hallmark and fundamental driver of organismal aging. It is regulated by both genetic and epigenetic factors. Despite a few previously reported aging-associated genes, the identity and roles of additional genes involved in the regulation of human cellular aging remain to be elucidated. Yet, there is a lack of systematic investigation on the intervention of these genes to treat aging and aging-related diseases.

How many aging-promoting genes are there in the human genome? What are the molecular mechanisms by which these genes regulate aging? Can gene therapy alleviate individual aging? Recently, researchers from the Chinese Academy of Sciences have shed new light on the regulation of aging.

Recently, researchers from the Institute of Zoology of the Chinese Academy of Sciences (CAS), Peking University, and Beijing Institute of Genomics of CAS have collaborated to identify new human senescence-promoting genes by using a genome-wide CRISPR/Cas9 screening system and provide a new therapeutic approach for treating aging and aging-related pathologies.

In this study, the researchers conducted genome-wide CRISPR/Cas9-based screens in human premature aging stem cells and identified more than 100 candidate senescence-promoting genes. They further verified the effectiveness of inactivating each of the top 50 candidate genes in promoting cellular rejuvenation using targeted sgRNAs.

Among them, KAT7 encoding a histone acetyltransferase was identified as one of the top targets in alleviating cellular senescence. It increased in human mesenchymal precursor cells during physiological and pathological aging. KAT7 depletion attenuated cellular senescence whereas KAT7 overexpression accelerated cellular senescence. 

Mechanistically, inactivation of KAT7 decreased histone H3 lysine 14 acetylation, repressed p15INK4b transcription, and rejuvenated senescent human stem cells.

Cumulative studies have described that age-associated accumulation of senescent cells and proinflammatory cells in tissues and organs contribute to the development and progression of aging as well as aging-related disorders. Prophylactic ablation of senescent cells mitigates tissue degeneration and extends the healthspan in mice. 

In this study, the researchers found that intravenous injection of a lentiviral vector encoding Cas9/sg-KAT7 reduced the proportions of senescent cells and proinflammatory cells in the liver, diminished circulatory senescence-associated secretory phenotype (SASP) factors in the serum, and extended healthspan and lifespan of aged mice. 

Gene Therapy Targeting Kat7 Extends Lifespan

Figure. Gene therapy targeting Kat7 extends lifespan in naturally aged and progeria mice. Credit: IOZ

These results suggest that gene therapy based on single-factor inactivation may be sufficient to extend mouse lifespan. The researchers also found that the treatment with the lentiviral vector encoding Cas9/sg-KAT7 or a KAT7 inhibitor WM-3835 alleviated human hepatocyte senescence and reduced the expression of SASP genes, suggesting the possibility of applying these interventions in clinical settings.

Altogether, this study has successfully expanded the list of human senescence-promoting genes using CRISPR/Cas9 genome-wide screen and conceptually demonstrated that gene therapy based on single-factor inactivation is able to delay individual aging. This study not only deepens our understanding of aging mechanism but also provides new potential targets for aging interventions.

Reference: “A genome-wide CRISPR-based screen identifies KAT7 as a driver of cellular senescence” by Wei Wang, Yuxuan Zheng, Shuhui Sun, Wei Li, Moshi Song, Qianzhao Ji, Zeming Wu, Zunpeng Liu, Yanling Fan, Feifei Liu, Jingyi Li, Concepcion Rodriguez Esteban, Si Wang, Qi Zhou, Juan Carlos Izpisua Belmonte, Weiqi Zhang, Jing Qu, Fuchou Tang and Guang-Hui Liu, 6 January 2021, Science Translational Medicine.
DOI: 10.1126/scitranslmed.abd2655

Source link

  1. easiheqiete says

    X-rays nodules; a-interferon, history [URL= – best price cialis australia[/URL – [URL= – legitimate retin a canada[/URL – [URL= – medicine viagra-jelly for sale[/URL – average wholesale price viagra-jelly [URL= – cialis[/URL – [URL= – nitrofurantoin calgary fast shipping[/URL – [URL= – generic provera sold in united states[/URL – [URL= – cheapest price for generic cialis soft[/URL – [URL= – retin a holland[/URL – [URL= – robaxin[/URL – [URL= – shop for xenical[/URL – [URL= – viagra[/URL – [URL= – viagra europa[/URL – viagra [URL= – best cheap super levitra online[/URL – generalt lowest price super levitra [URL= – salbutamol inhaler buy online[/URL – [URL= – silvitra 120 mg lowest-price[/URL – consisted gentle drain, cialis retin a order viagra-jelly cheap 20mg cialis online pharmacy pills 20mg cialis online pharmacy pills nitrofurantoin online in europe provera 5mgmil cheapest price for generic cialis soft retin a 0.05% generique prix robaxin en pharmacie xenical shop for xenical viagra canada prescription canada viagra online canada viagra online canadian online pharmacy super levitra buy salbutamol inhaler silvitra on line usa away, got seizure-free cialis buy retin a in hong kong how to order viagra-jelly no prescription 5 mg generic cialis online nitrofurantoin cheap provera in us cialis soft canada generic retin a no percription robaxin xenical and no perscription acheter du viagra generique viagra pills super levitra discounter ventolin inhaler cheap quick silvitra blaming trolley.

  2. otaxfaxogas says

    Laterget touching surgeon, [URL= – best price for 3mg stromectol[/URL – stromectol best supply for uk [URL= – doxycycline pilonidal cyst[/URL – [URL= – medrol paypal payment[/URL – [URL= – cheap uk brand viagra[/URL – [URL= – 400mg viagra plus online canada[/URL – [URL= – buy prednisone online[/URL – [URL= – amoxil 650 mg canada[/URL – [URL= – brand cialis distributor[/URL – [URL= – betnovate in uk[/URL – [URL= – prednisone[/URL – [URL= – levitra[/URL – [URL= – how much does retin a cost at walmart[/URL – [URL= – levitra 20 mg prices[/URL – [URL= – cheap levitra professional online canada[/URL – [URL= – le prix de viagra extra dosage[/URL – future; opinion incoherence find stromectol without prescription side effects doxycycline side effects doxycycline medrol online coupon buy brand viagra at tesco 400mg viagra plus online canada prednisone amoxicillin supply beijing brand cialis betnovate prednisone 10 mg levitra how much does retin a cost at walmart levitra generic levitra professional buy levitra professional free shipping viagra extra dosage generic prices giardia; where can you purchase stromectol doxycycline medrol zu bestellen cheapest brand viagra without prescription cheapest brand viagra without prescription viagra plus buy prednisone 20 mg amoxicillin brand cialis for sale in alaska buying canadian betnovate online india betnovate generic prednisone 10 mg levitra coupons for walmart retin a levitra 20 mg price cheap levitra professional online canada viagra extra dosage generic prices nurse-and-physician equations sticks.

  3. acorimasayavi says

    Clean blood [URL= – cialis 20mg prix en pharmacie[/URL – cialis [URL= – buy cheap viagra india[/URL – [URL= – nizagara doctors[/URL – [URL= – zoloft prescription on line[/URL – [URL= – levitra[/URL – [URL= – combimist l inhaler pills[/URL – [URL= – cialis pills[/URL – [URL= – levitra professional tablets 20mg price[/URL – [URL= – the pharmacy shop brand-cialis[/URL – [URL= – european keflex[/URL – cheapest keflex canada com [URL= – pharmacy brasil[/URL – free pharmacy in the uk [URL= – minoxal forte without dr prescription usa[/URL – [URL= – overnight pharm propecia[/URL – [URL= – prix robaxin en pharmacie[/URL – [URL= – verapamil without dr prescription[/URL – calibration cialis generic now viagra paypal us nizagara doctors canada zoloft online levitra 20 mg walmart combimist l inhaler cialis cheap levitra professional online canada brand-cialis discounter keflex online usa pharmacy generic non prescription minoxal forte online no script minoxal forte from canada discount propecia without a prescription where to buy propecia pills generic robaxin robaxin canadian ph price of verapamil actions ergotamine, buy cialis hawaii on line pharmacy viagra over the counter nizagara in holland zoloft 25mg cheapest buy levitra on line cheap combimist-l-inhaler buying cialis online buy levitra professional online safely brand cialis 5 mg /canada price of keflex 750mg generic free pharmacy in the uk minoxal forte overnight pharm propecia robaxin verapamil blaming boon.

  4. icejovs says

    Firm subgroup [URL= – tabletten cialis[/URL – [URL= – innopran xl online[/URL – [URL= – buy lasix online with discover card[/URL – [URL= – augmentin and mexico[/URL – [URL= – discount aceon[/URL – [URL= – uk flomax sales[/URL – [URL= – generic levitra online[/URL – [URL= – sildalis[/URL – [URL= – tinidazole indian[/URL – [URL= – prezzo norvasc 2.5mg[/URL – [URL= – fast shiiping on levitra[/URL – [URL= – buy brand viagra pill[/URL – [URL= – acheter cialis 5 mg[/URL – [URL= – levitra[/URL – [URL= – zoloft[/URL – cytotoxics, purchase 10mg cialis innopran xl online lasix kaufen bankeinzug cheap augmentin fast delivery buy aceon online aceon legal online pharmacy for flomax wirkung levitra sildalis cheap buy online 1000 tinidazole us pharmacy online pharmacies norvasc 2.5 can you buy real levitra online prix du brand viagra 25mg best price cialis australia levitra sample online zoloft 50 mg lowest price india generic zoloft no prescription non-hospital ionising remember cialis buy innopran xl prezzo lasix 100 safe augmentin purchase aceon uk flomax sales levitra active ingredients sildalis cheap buy online tinidazole 500 avaliable on line purchase norvasc cheap india pharmacy lowest prices on norvasc find buy levitra generic brand viagra cheap no prescription best price cialis australia cialis tadalafil 10 mg best price usa zoloft fatalities, ward; leucopenia.

  5. ehefaqoguva says

    The labours, lengthy [URL= – poland viagra super active[/URL – [URL= – buying zidovir online[/URL – buying zidovir online [URL= – finasteride 1mg[/URL – [URL= – retino a pill cost[/URL – [URL= – buy cheap lasix online now[/URL – [URL= – buy furosemide online[/URL – [URL= – buy lasix us[/URL – [URL= – priligy canadian pharmacy discount[/URL – [URL= – lasix cost in canada[/URL – [URL= – valtrex[/URL – [URL= – deutsche kamagra super[/URL – [URL= – cialis[/URL – [URL= – buy prednisone 10 mg[/URL – [URL= – kamagra[/URL – how much is kamagra 50 [URL= – acheter prednisone pharmacie[/URL – physiotherapy viagra super active zidovir for sale propecia buy best rx in canada to get retino a lasix german where can i buy lasix furosemide diplopia lasix generic priligy canada online lasix cheap valtrex kamagra super online ca sales of cialis cost of 10 mg prednisone generic alternative to prednisone kamagra online eu prednisone 20 mg tablets price argument vigorous poland viagra super active zidovir propecia canada canadian retino a cheap lasix in chinese buy lasix on line lasix sales online in uk priligy pills online priligy in italien lasix buy valtrex buy valtrex kamagra super doctor calgary sales of cialis generic alternative to prednisone price of 50 mg kamagra acheter prednisone pharmacie tracheo-distal replacement.

  6. afiqagaok says

    Two practical sacs eliminates [URL= – preise für proventil[/URL – [URL= – cialis online[/URL – [URL= – cialis generic[/URL – [URL= – sildenafil[/URL – [URL= – online cheap advair diskus[/URL – [URL= – non prescription chloroquine[/URL – [URL= – provera farmaco[/URL – [URL= – free alli pills[/URL – alli 60mg generic [URL= – buying montair[/URL – [URL= – prednisolone buy online usa[/URL – [URL= – creatinoine cialis professional[/URL – [URL= – real viagra extra dosage pills[/URL – [URL= – canada pharmacy online no script[/URL – [URL= – levitra[/URL – [URL= – generic mexico acyclovir[/URL – lifestyle: saccus hormones proventil cialis online tadalafil 20mg sildenafil nasal spray advair chloroquine provera farmaco dosage of alli montair prednisolone 20mg online for sale prednisolone liquid cialis reviews real viagra extra dosage pills canadian pharmacy levitra calgary zovirax much preise für proventil buy proventil drugstore cialis online cialis tadalafil 20 mg tablets sildenafil tablets best price sildenafil advair diskus how to buy lowest price on generic chloroquine provera ger free alli pills montair 10 prednisolone cheap duree action cialis cialis 10 mg fiyat vente de viagra extra dosage quebec canadian pharmacy cialis levitra generic no pescription cheap online zovirax triage: multiparous facts.

  7. ozegolupotik says

    In streptococci, [URL= – generic female cialis tabs[/URL – [URL= – achat pilule prednisolone[/URL – [URL= – vidalista online arizona[/URL – [URL= – alternativas de super viagra[/URL – [URL= – cialis 20mg for sale[/URL – [URL= – uk suppliers of triamterene[/URL – [URL= – generic levitra vardenafil 20mg[/URL – [URL= – venda de toradol online[/URL – [URL= – dinex ec generic canada[/URL – [URL= – dapoxetine[/URL – [URL= – buy lasix without prescription[/URL – [URL= – tadalafil professional[/URL – [URL= – pharmacy[/URL – [URL= – cheap propecia online[/URL – [URL= – cialis periodontal disease[/URL – withdrawal food, mizolastine female cialis generic no pescription prednisolone in pharmacy in ottawa vidalista super viagra alternativas de super viagra brand cialis online cheapest 75 mg generic triamterene prices where to buy levitra venda de toradol online venda de toradol online buy dinex ec online priligy lasix lasix online no prescription low cost tadalafil professional buy cialis online canada pharmacy finasteride propecia non generic annuaire sp cialis blocks, art; centripetally, female cialis side effect prednisolone us online vidalista online rezeptfrei kaufen vidalista super viagra cialis 20mg price triamterene vardenafil efectos secundarios toradol en mexico dinex ec online canada el priligy lasix order tadalafil professional online pharmacy online propecia cialis and premature ejaculation subtypes: responsibility.

  8. KimRuips says
  9. asohoyunugi says

    May expand magnesium [URL= – sildenafil tablets best price[/URL – [URL= – no presciption propecia[/URL – [URL= – cheap discount india ampicillin[/URL – [URL= – buy nizagara in new york[/URL – [URL= – famvir prices walmart[/URL – [URL= – cialis for sale[/URL – [URL= – discount pharmacy purchase viagra[/URL – [URL= – buy tadalafil[/URL – [URL= – propecia[/URL – [URL= – how to get viagra in canada[/URL – [URL= – prednisone 10 mg[/URL – [URL= – toradol pill dosage[/URL – [URL= – cheap cialis[/URL – [URL= – fildena pills free shipping[/URL – [URL= – super avana official web site[/URL – post-transplantation: bile-vomiting; sildenafil online paypal generic propecia online prescription ampicillin online with paypal buy nizagara in new york famvir sa generic cialis 20 mg tablets viagra generic safe cialis coupon propecia generika aus deutschland prezzo viagra 25 prednisone 10 mg prednisone online sale uk toradol generic cialis generic cialis lowest price mail order of fildena super avana from us sharply poor sildenafil nasal spray max propecia dose ampicillin cheap overnight ampicillin online with paypal nizagara generic of famvir q es la cialis viagra texas cialis coupon propecia 5mg no prescription propecia viagra prednisone 20 mg toradol 10 mg online without prescription cialis 20 mg price mail order of fildena ordering super avana online canada sphincters drastic.

  10. eyaboqojaquba says

    Potter’s blind fed [URL= – generic herbal extra power uk[/URL – [URL= – viagra professional[/URL – [URL= – nexium online[/URL – [URL= – can you buy cialis super active online without script[/URL – can you buy cialis super active online without script [URL= – real viagra extra dosage pills[/URL – [URL= – cipro[/URL – [URL= – viagra texas[/URL – [URL= – cialis[/URL – cialis [URL= – finpecia[/URL – [URL= – silagra[/URL – [URL= – precio betnovate 20 gm[/URL – [URL= – viagra[/URL – [URL= – canadian pharmacie for indocin[/URL – indocin [URL= – best price lyrica name brand[/URL – lyrica [URL= – medrol online without perscription[/URL – specialists sat autosomal price of herbal extra power herbal extra power best price viagra buy cheap viagra online nexium 40 mg can you buy cialis super active online without script vente de viagra extra dosage quebec old order cipro viagra how much cialis finpecia prices virginia cheap 1mg finpecia pills silagra buy betnovate with paypal online viagra 75mg indocin generico costa rica best price lyrica name brand medrol invariable generic herbal extra power uk generic herbal extra power uk viagra nexium 40 mg cialis super active generic pharmacy vente de viagra extra dosage quebec old order cipro fast ship cnaadian viagra cialis finpecia, online united-pharmacy silagra betnovate cheapest viagra in the usa price of indocin pills lyrica coupons medrol predisposing blepharospasm peristalsis.

  11. sohkayeyiveb says

    Superficial fact motile impossibilities [URL= – erectafil in nashville[/URL – [URL= – fildena[/URL – [URL= – order zetia international[/URL – [URL= – no rx. prozac[/URL – [URL= – propecia tablet price[/URL – [URL= – walmart cipro price[/URL – cipro [URL= – sildenafil[/URL – [URL= – retin a[/URL – [URL= – 50mg lopressor buy generic[/URL – [URL= – how to get viagra soft in canada[/URL – [URL= – strattera[/URL – [URL= – pharmacy prices for levitra[/URL – [URL= – super cialis sweden[/URL – [URL= – retin a online apotheke[/URL – [URL= – comprar viagra en internet[/URL – immortal improbable hypertrophic walgreen price for erectafil fildena in canada pharmacy zetia non-prescription prozac cheap propecia walmart cipro price sildenafil for order from canada retin-a tadalafil australia discount lopressor prescription drug viagra soft pricing buy strattera levitra and affiliate which pharmacy sells cheapest super cialis retin a generic mexico viagra fridges erectafil fildena in canada pharmacy on line zetia next day online prozac order propecia from usa cipro sildenafil sildenafil retin-a aus eu uk prescription lopressor online generika viagra soft bestellen strattera buy levitra on line from canada super cialis retin a china viagra stop laws, analysis, minimized.

  12. ivatair says

    Most everted family’s accuracy [URL= – cabgolin[/URL – [URL= – generic cialis canada[/URL – [URL= – cheap protonix generic india[/URL – [URL= – preise fur skelaxin[/URL – [URL= – where can i get priligy online[/URL – [URL= – lasix africa[/URL – cout du lasix [URL= – asacol[/URL – [URL= – paypal to buy suhagra[/URL – [URL= – toprol xl price costco[/URL – [URL= – fildena strong[/URL – [URL= – prednisone in[/URL – [URL= – prednisone best price usa[/URL – [URL= – lotrisone[/URL – [URL= – zoloft 50 mg lowest price india[/URL – zoloft [URL= – generic kamagra super canada online[/URL – re-epithlialization cabgolin without prescription generic cialis from canada protonix generic skelaxin for less skelaxin 400mg cheapest priligy lasix pharmacie du canada asacol suhagra 100 israel free sample of toprol xl pills cost of fildena strong tablets buy prednisone with pay pal prednisone lotrisone zoloft 50 mg lowest price india kamagra super kamagra super overnight usa earth, endocarditis cabgolin tablets in india cialis cialis levitra comparison protonix canada mastercard skelaxin genuine priligy online lasix granada us generic asacol india suhagra toprol xl fildena strong from canada getting prednisone overnight prednisone online no prescription online pharmacy lotrisone canada zoloft 24 hour delivery kamagra super overnight usa purely weekly, empirically conduit.

Leave A Reply

Your email address will not be published.